Research Technologist II Ophthalmology

JOB REQUIREMENTS: Summary The applicant will join the Miesfeld laboratory at the Medical College of Wisconsin Eye Institute and perform research tasks under the guidance of Dr. Joel B. Miesfeld. The research performed in the Miesfeld lab explores the gene regulatory mechanisms controlling retinal development, disease and regeneration, with an emphasis on retinal ganglion cells (RGCs), the pathogenic target in Glaucoma and conduit connecting the eye to the brain. The laboratory combines a variety of techniques (biochemistry, histochemistry, molecular biology, microscopy, CRISPR/Cas9 genome editing, etc.) and model systems (mouse, zebrafish, tissue culture) to elucidate the mechanisms responsible for retinal homeostasis. As a member of the Miesfeld lab the applicant will assist research staff in the everyday running of the lab and provide research support to further our understanding of eye development, disease, and vision loss prevention. Primary Responsibilities Assist in the breeding and PCR genotyping of mutant/transgenic mice and zebrafish. Conduct molecular biology work, including plasmid tool assembly via cloning. Collect and process tissue for bio- and histochemical analysis and subsequent data collection (microscopy, protein detection, high-throughput DNA/RNA sequencing). Carry out routine laboratory duties, including the washing and sterilizing of glassware and equipment, disposal of laboratory waste, ordering and inventory of laboratory supplies, and coordinating shipping of materials to and from research collaborators. Maintain complete, accurate records and summaries of all experimental results and data generated. Assist with the evaluation, interpretation and assessment of experimental data and quality control assays. Participate in meetings with principal investigator and research staff. Complete all necessary formal training requirements (e.g. health and safety training) promptly. Oversee the training of other lab personnel Other duties assigned Knowledge - Skills - Abilities Knowledge of biology, chemistry, mathematics, documentation, and records management. Data utilization, complex problem solving, critical thinking, resource management, and writing skills. Qualifications Appropriate experience may be substituted for education an on equivalent basis. Minimum Required Education: Bachelor's degree Minimum Required Experience: 1 year of mouse experience, which includes handling, colony management, tissue collection, and genotyping. Mouse experience can be substituted for zebrafish experience. Standard molecular biology techniques such as molecular cloning and... For full info follow application link. EEO/AA Minorities/Females/Vets/Disability The Medical College of Wisconsin is an affirmative action/equal opportunity employer and does not discriminate in hiring or employment on the basis of age, sex, race, color, religion, national origin, veteran status, disability or sexual orientation. ***** APPLICATION INSTRUCTIONS: Apply Online: ipc.us/t/7A175B9E23234389 Qualified females, minorities, and special disabled veterans and other veterans are encouraged to apply.